Our scientific team provides flexible, high-sensitivity support across advanced microscopy, multi-color flow cytometry, plate-based ELISA, and multiplex FluoroSpot/ELISpot testing. We work alongside you to deliver tailored assay design, panel validation, and reliable execution from routine screening to custom protocol development. These robust workflows ensure reproducible data and clear, actionable insights to accelerate your translational and pharmaceutical breakthroughs.
We provide expert imaging for a range of biological samples and experimental needs—from routine visual checks to fluorescence and confocal imaging for more detailed visualisation. Our setup supports widefield epifluorescence, spinning-disk confocal imaging, and Differential Interference Contrast (DIC) for enhanced contrast, alongside standard tools for image processing and analysis.
Filter cubes: DAPI, GFP, RFP, Cy5
Laser lines: 365 nm, 488 nm, 561 nm, 640 nm
ELISpot (Enzyme-Linked Immunospot) is a highly sensitive immunoassay used to detect and quantify cytokine or other analyte secretion from individual cells. FluoroSpot builds on the same principle, using fluorophore-labelled detection antibodies to assess multiple analytes simultaneously from a single cell. Together, these assays provide robust options for immune monitoring, immunogenicity assessment and functional characterisation of cellular responses.
In both assay formats, secreted analytes are captured locally around responding cells, with each spot corresponding to an individual secreting cell. ELISpot provides highly sensitive single-analyte readout, while FluoroSpot enables multiplex detection to characterise low-frequency and multifunctional responding cells in greater detail.
We provide ELISpot services using Virax Biolabs’ validated kit formats or custom workflows tailored to your study needs. Available ELISpot configurations include pre-coated plates, uncoated plates and 8-well strips.
For antigen-driven studies, assays can be combined with Virax ImmuneSelect peptide pools for defined stimulation and consistent readout.
We provide FluoroSpot services using custom workflows designed around your study requirements. Assays can be adapted for different sample types, stimulation strategies and multiplex targets. For antigen-driven applications, workflows can also be integrated with Virax ImmuneSelect peptide pools.
Readout is performed using fluorescence-capable AID, CTL and Eazy Reader systems, with automated spot detection and expert manual review.
We provide flexible analytical flow cytometry support, from straightforward immunophenotyping through to functional readouts such as viability, apoptosis, proliferation and activation marker analysis. Whether you already have a defined panel or need input on feasibility, fluorophore selection or gating strategy, we can help shape a practical workflow that fits your experimental objectives and available instrument parameters.
Our ELISA services provide high-sensitivity detection and quantification of target analytes within liquid samples, including serum, plasma, and cell culture supernatants. We support a diverse range of plate-based immunoassay formats tailored to specific target biology, antibody availability, and complex sample matrices to deliver reproducible data for proteins, peptides, cytokines, hormones, and other soluble biomarkers.
Our scientific team provides comprehensive technical oversight throughout the workflow, delivering expert guidance on sample preparation, matrix dilution planning, standard curve configuration, and assay optimization to guarantee rigorous analytical precision.
Our RT-qPCR (Reverse Transcription Quantitative PCR) services support sensitive nucleic acid detection for projects requiring gene expression analysis, pathogen detection, target validation or molecular profiling. The approach is well suited for rapid, specific and quantitative measurement of RNA or DNA targets from limited or complex biological material.
Get in touch to find out how we can support your research needs.
