Cat #:
2153

ELISpot Human IL-2, uncoated

£ 110.00

Shipped within 2-3 days
Product Details
Application: ELISpot
Analyte: IL-2
Reactivity: Human
Storage: Store plates and reagents between 2 and 8°C
Shelf Life: 12 months
Peptide Pools​: No peptide pool included - plate and reagents only
Plates​: ELISpot IL-2 capture antibody
Detection mAb​​: Biotinylated recombinant IL-2 antibody
Enzyme conjugate​: Streptavidin-ALP (Alkaline Phosphatase)
Substrate: BCIP/NBT Ready-to-use solution
Blocking agent: Bovine Serum Albumin (BSA)
IL-2 is a multifunctional cytokine involved in both innate and adaptive immunity. IL-2 is primarily produced by activated CD4+ T cells but can also be produced by CD8+ T cells, dendritic cells, NK cells, and certain subsets of B cells during immune responses. As a proinflammatory cytokine, IL-2 plays a significant role in the activation and proliferation of various immune cells, facilitating their growth, differentiation, and survival.

 

IL-2 functions as a T cell growth factor and can also increase the cytolytic activity of NK cells. Additionally, it plays a crucial role in promoting proliferation of activated B cells, which subsequently leads to increased immunoglobulin production. This effect enhances the immune response against a wide array of pathogens by promoting the expansion and activation of effector immune cells. In addition to its role in activating immune cells, IL-2 is crucial for the induction of regulatory T cells, which help maintain immune tolerance and prevent autoimmune reactions. The cytokine also stimulates the production of other cytokines and growth factors, contributing to the overall coordination and amplification of immune responses across different cell types and pathways.

The Human IL-2 Enzyme-Linked ImmunoSpot (ELISpot) assay is a highly sensitive immunological technique that detects cytokine secretion at the single-cell level. Unlike methods such as ELISA or RT-PCR, which measure cytokine or cytokine-related signals at the population level, ELISpot enables identification and quantification of the frequency of individual cytokine-secreting cells. This assay can be used assess cellular immune responses by determining the frequency of stimulated effector cells that secrete IL-2. Non-coated kits are a cost-effective choice that provide greater flexibility to support different experimental requirements.

 

Description

IL-2 is a multifunctional cytokine involved in both innate and adaptive immunity. IL-2 is primarily produced by activated CD4+ T cells but can also be produced by CD8+ T cells, dendritic cells, NK cells, and certain subsets of B cells during immune responses. As a proinflammatory cytokine, IL-2 plays a significant role in the activation and proliferation of various immune cells, facilitating their growth, differentiation, and survival.

 

IL-2 functions as a T cell growth factor and can also increase the cytolytic activity of NK cells. Additionally, it plays a crucial role in promoting proliferation of activated B cells, which subsequently leads to increased immunoglobulin production. This effect enhances the immune response against a wide array of pathogens by promoting the expansion and activation of effector immune cells. In addition to its role in activating immune cells, IL-2 is crucial for the induction of regulatory T cells, which help maintain immune tolerance and prevent autoimmune reactions. The cytokine also stimulates the production of other cytokines and growth factors, contributing to the overall coordination and amplification of immune responses across different cell types and pathways.

Application

The Human IL-2 Enzyme-Linked ImmunoSpot (ELISpot) assay is a highly sensitive immunological technique that detects cytokine secretion at the single-cell level. Unlike methods such as ELISA or RT-PCR, which measure cytokine or cytokine-related signals at the population level, ELISpot enables identification and quantification of the frequency of individual cytokine-secreting cells. This assay can be used assess cellular immune responses by determining the frequency of stimulated effector cells that secrete IL-2. Non-coated kits are a cost-effective choice that provide greater flexibility to support different experimental requirements.

 

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Frequently Asked Questions

Virax Biolabs develops and provides immunology-profiling solutions, with a focus on T cell diagnostics and immune monitoring. Their offerings include research-use products (e.g., peptide pools, ELISpot/FluoroSpot kits, high-affinity antibodies) as well as custom immunology services (such as recombinant antibody engineering, peptide synthesis and protein sequencing).

Virax Biolabs offers a comprehensive range of products to support immunology research and diagnostic development. Their primary product categories include pathogen-specific peptide pools that enable T cell stimulation and immune-response analysis, ELISpot and FluoroSpot kits designed for measuring cytokine secretion and other immune markers, and high-affinity antibodies suitable for use in assays such as ELISA, flow cytometry, and ELISpot. Together, these products provide researchers with precise tools for profiling immune responses in various disease and vaccine studies.

Virax Biolabs provides a range of custom immunology services to meet the diverse needs of academic and industry clients. These services include recombinant antibody engineering, which involves antigen discovery, sequencing, formatting, and bio-conjugation. They also offer custom peptide synthesis and protein sequencing using advanced mass spectrometry, as well as laboratory services that support ELISA, ELISpot, FluoroSpot, and flow cytometry assays. These custom services are designed to help researchers accelerate discovery and optimize immune-response studies.

Virax Biolabs primarily serves researchers and organizations engaged in immunology, infectious disease, and vaccine development. Their customer base includes academic laboratories, biotechnology companies, and pharmaceutical firms that require high-quality immune-profiling tools and diagnostic support. By providing both off-the-shelf research products and tailored immunology services, Virax Biolabs enables scientists to investigate T cell activity, evaluate vaccine efficacy, and develop new diagnostic platforms.

Virax Biolabs’ headquarters is located at BioCity Glasgow, Bo’Ness Road, Newhouse, Lanarkshire, ML1 5UH, United Kingdom. Inquiries can be made through the “Contact” section of their website at viraxbiolabs.com.

Many of Virax Biolabs’ products, including peptide pools and ELISpot kits, are intended for research use in immune-profiling and in-vitro studies. However, the company is also developing in-vitro diagnostic (IVD) platforms and T cell diagnostics for applications such as chronic inflammation and immune exhaustion. The intended use and regulatory status vary by product, so customers should refer to the specific product details or contact the company directly for clarification.

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