ELISpot Human IL-6
Cat #:
2157

ELISpot Human IL-6

£ 110.00

Shipped within 2-3 days
Product Details
Application: ELISpot
Analyte: IL-6
Reactivity: Human
Storage: Store plates and reagents between 2 and 8°C
Shelf Life: 12 months
Peptide Pools​: No peptide pool included - plate and reagents only
Plates​: ELISpot IL-6 capture antibody
Detection mAb​​: Biotinylated recombinant IL-6 antibody
Enzyme conjugate​: Streptavidin-ALP (Alkaline Phosphatase)
Substrate: BCIP/NBT Ready-to-use solution
Blocking agent: Bovine Serum Albumin (BSA)
Interleukin-6 (IL-6) is a multifunctional cytokine involved in immune regulation, inflammation, and hematopoiesis. It is produced by a wide range of cells, including T cells, B cells, macrophages, fibroblasts, and endothelial cells in response to infection, injury, or immune activation. IL-6 plays a central role in the acute phase response and supports the transition from innate to adaptive immunity. In the adaptive immune system, IL-6 promotes the maturation of B cells into antibody-secreting plasma cells and contributes to the expansion of effector T cells. It also plays a role in the differentiation of Th17 cells and can modulate the activity of regulatory T cells, thereby influencing the balance between pro- and anti-inflammatory responses. Due to its broad immunological functions, IL-6 is a key cytokine of interest in immune monitoring and is commonly used as a readout in assays assessing inflammatory or immune activation states.    

The Human IL-6 Enzyme-Linked ImmunoSpot (ELISpot) assay is a highly sensitive method used to detect and quantify individual cells that secrete specific cytokines or other soluble molecules. This assay is utilized in immunology to monitor cellular immune responses at the single-cell level and reliably detects and measures human IL-6 secretion by stimulated effector cells. Non-coated kits are a cost-effective choice that provide greater flexibility to support different experimental requirements.

 

Description

Interleukin-6 (IL-6) is a multifunctional cytokine involved in immune regulation, inflammation, and hematopoiesis. It is produced by a wide range of cells, including T cells, B cells, macrophages, fibroblasts, and endothelial cells in response to infection, injury, or immune activation. IL-6 plays a central role in the acute phase response and supports the transition from innate to adaptive immunity. In the adaptive immune system, IL-6 promotes the maturation of B cells into antibody-secreting plasma cells and contributes to the expansion of effector T cells. It also plays a role in the differentiation of Th17 cells and can modulate the activity of regulatory T cells, thereby influencing the balance between pro- and anti-inflammatory responses. Due to its broad immunological functions, IL-6 is a key cytokine of interest in immune monitoring and is commonly used as a readout in assays assessing inflammatory or immune activation states.    

Application

The Human IL-6 Enzyme-Linked ImmunoSpot (ELISpot) assay is a highly sensitive method used to detect and quantify individual cells that secrete specific cytokines or other soluble molecules. This assay is utilized in immunology to monitor cellular immune responses at the single-cell level and reliably detects and measures human IL-6 secretion by stimulated effector cells. Non-coated kits are a cost-effective choice that provide greater flexibility to support different experimental requirements.

 

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Frequently Asked Questions

Virax Biolabs develops and provides immunology-profiling solutions, with a focus on T cell diagnostics and immune monitoring. Their offerings include research-use products (e.g., peptide pools, ELISpot/FluoroSpot kits, high-affinity antibodies) as well as custom immunology services (such as recombinant antibody engineering, peptide synthesis and protein sequencing).

Virax Biolabs offers a comprehensive range of products to support immunology research and diagnostic development. Their primary product categories include pathogen-specific peptide pools that enable T cell stimulation and immune-response analysis, ELISpot and FluoroSpot kits designed for measuring cytokine secretion and other immune markers, and high-affinity antibodies suitable for use in assays such as ELISA, flow cytometry, and ELISpot. Together, these products provide researchers with precise tools for profiling immune responses in various disease and vaccine studies.

Virax Biolabs provides a range of custom immunology services to meet the diverse needs of academic and industry clients. These services include recombinant antibody engineering, which involves antigen discovery, sequencing, formatting, and bio-conjugation. They also offer custom peptide synthesis and protein sequencing using advanced mass spectrometry, as well as laboratory services that support ELISA, ELISpot, FluoroSpot, and flow cytometry assays. These custom services are designed to help researchers accelerate discovery and optimize immune-response studies.

Virax Biolabs primarily serves researchers and organizations engaged in immunology, infectious disease, and vaccine development. Their customer base includes academic laboratories, biotechnology companies, and pharmaceutical firms that require high-quality immune-profiling tools and diagnostic support. By providing both off-the-shelf research products and tailored immunology services, Virax Biolabs enables scientists to investigate T cell activity, evaluate vaccine efficacy, and develop new diagnostic platforms.

Virax Biolabs’ headquarters is located at BioCity Glasgow, Bo’Ness Road, Newhouse, Lanarkshire, ML1 5UH, United Kingdom. Inquiries can be made through the “Contact” section of their website at viraxbiolabs.com.

Many of Virax Biolabs’ products, including peptide pools and ELISpot kits, are intended for research use in immune-profiling and in-vitro studies. However, the company is also developing in-vitro diagnostic (IVD) platforms and T cell diagnostics for applications such as chronic inflammation and immune exhaustion. The intended use and regulatory status vary by product, so customers should refer to the specific product details or contact the company directly for clarification.

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