ELISpot Human IL-8 (CXCL8), 12x8 well strip
Cat #:
2226

ELISpot Human IL-8 (CXCL8), 12×8 well strip

£ 180.00

Shipped within 2-3 days
Product Details
Application: ELISpot
Analyte: IL-8
Reactivity: Human
Storage: Store plates and reagents between 2 and 8°C
Shelf Life: 12 months
Peptide Pools​: No peptide pool included - plate and reagents only
Plates​: 12x8 well ELISpot plate precoated with IL-8 capture antibody
Detection mAb​​: Biotinylated recombinant IL-8 antibody
Enzyme conjugate​: Streptavidin-ALP (Alkaline Phosphatase)
Substrate: BCIP/NBT Ready-to-use solution
Blocking agent: Bovine Serum Albumin (BSA)
Interleukin-8 (IL-8 / CXCL8) is a pro-inflammatory chemokine that plays a key role in the recruitment and activation of immune cells, particularly neutrophils. It is secreted by multiple cell types, including monocytes, macrophages, epithelial cells, and endothelial cells, in response to inflammatory stimuli or microbial products. IL-8 is best known for directing neutrophil chemotaxis to sites of infection or tissue injury and enhancing their activation and degranulation. In addition to its role in innate immunity, IL-8 can also act on subsets of T cells, particularly memory and activated T cells that express CXCR1 or CXCR2 promoting their migration into inflamed tissues and contributing to the shaping of local immune responses. Due to its role in immune cell recruitment, inflammation, and tissue remodeling, IL-8 is widely used as a biomarker in studies of infection, inflammation, and tumor immunology.    

The Human IL-8 (CXCL8) Enzyme-Linked ImmunoSpot (ELISpot) assay is a highly sensitive method used to detect and quantify individual cells that secrete specific cytokines or other soluble molecules. This assay is utilized in immunology to monitor cellular immune responses at the single-cell level and reliably detects and measures human IL-8 secretion by stimulated effector cells. Non-coated kits are an economical option that also offer more flexibility to meet a variety of experimental needs. 8-well strip (12×8) ELISPOT plates offer a flexible, cost-effective alternative to full 96-well plates by allowing smaller-scale assays, reduced reagent use, and easier handling for variable experimental setups.

 

Description

Interleukin-8 (IL-8 / CXCL8) is a pro-inflammatory chemokine that plays a key role in the recruitment and activation of immune cells, particularly neutrophils. It is secreted by multiple cell types, including monocytes, macrophages, epithelial cells, and endothelial cells, in response to inflammatory stimuli or microbial products. IL-8 is best known for directing neutrophil chemotaxis to sites of infection or tissue injury and enhancing their activation and degranulation. In addition to its role in innate immunity, IL-8 can also act on subsets of T cells, particularly memory and activated T cells that express CXCR1 or CXCR2 promoting their migration into inflamed tissues and contributing to the shaping of local immune responses. Due to its role in immune cell recruitment, inflammation, and tissue remodeling, IL-8 is widely used as a biomarker in studies of infection, inflammation, and tumor immunology.    

Application

The Human IL-8 (CXCL8) Enzyme-Linked ImmunoSpot (ELISpot) assay is a highly sensitive method used to detect and quantify individual cells that secrete specific cytokines or other soluble molecules. This assay is utilized in immunology to monitor cellular immune responses at the single-cell level and reliably detects and measures human IL-8 secretion by stimulated effector cells. Non-coated kits are an economical option that also offer more flexibility to meet a variety of experimental needs. 8-well strip (12×8) ELISPOT plates offer a flexible, cost-effective alternative to full 96-well plates by allowing smaller-scale assays, reduced reagent use, and easier handling for variable experimental setups.

 

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Frequently Asked Questions

Virax Biolabs develops and provides immunology-profiling solutions, with a focus on T cell diagnostics and immune monitoring. Their offerings include research-use products (e.g., peptide pools, ELISpot/FluoroSpot kits, high-affinity antibodies) as well as custom immunology services (such as recombinant antibody engineering, peptide synthesis and protein sequencing).

Virax Biolabs offers a comprehensive range of products to support immunology research and diagnostic development. Their primary product categories include pathogen-specific peptide pools that enable T cell stimulation and immune-response analysis, ELISpot and FluoroSpot kits designed for measuring cytokine secretion and other immune markers, and high-affinity antibodies suitable for use in assays such as ELISA, flow cytometry, and ELISpot. Together, these products provide researchers with precise tools for profiling immune responses in various disease and vaccine studies.

Virax Biolabs provides a range of custom immunology services to meet the diverse needs of academic and industry clients. These services include recombinant antibody engineering, which involves antigen discovery, sequencing, formatting, and bio-conjugation. They also offer custom peptide synthesis and protein sequencing using advanced mass spectrometry, as well as laboratory services that support ELISA, ELISpot, FluoroSpot, and flow cytometry assays. These custom services are designed to help researchers accelerate discovery and optimize immune-response studies.

Virax Biolabs primarily serves researchers and organizations engaged in immunology, infectious disease, and vaccine development. Their customer base includes academic laboratories, biotechnology companies, and pharmaceutical firms that require high-quality immune-profiling tools and diagnostic support. By providing both off-the-shelf research products and tailored immunology services, Virax Biolabs enables scientists to investigate T cell activity, evaluate vaccine efficacy, and develop new diagnostic platforms.

Virax Biolabs’ headquarters is located at BioCity Glasgow, Bo’Ness Road, Newhouse, Lanarkshire, ML1 5UH, United Kingdom. Inquiries can be made through the “Contact” section of their website at viraxbiolabs.com.

Many of Virax Biolabs’ products, including peptide pools and ELISpot kits, are intended for research use in immune-profiling and in-vitro studies. However, the company is also developing in-vitro diagnostic (IVD) platforms and T cell diagnostics for applications such as chronic inflammation and immune exhaustion. The intended use and regulatory status vary by product, so customers should refer to the specific product details or contact the company directly for clarification.

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