ELISpot Human TNF-α, 12x8 well strip
Cat #:
2248

ELISpot Human TNF-α, 12×8 well strip

£ 180.00

Shipped within 2-3 days
Product Details
Application: ELISpot
Analyte: TNF-α
Reactivity: Human
Storage: Store plates and reagents between 2 and 8°C
Shelf Life: 12 months
Peptide Pools​: No peptide pool included - plate and reagents only
Plates​: 12x8 well ELISpot plate precoated with TNF-α capture antibody
Detection mAb​​: Biotinylated recombinant TNF-α antibody
Enzyme conjugate​: Streptavidin-ALP (Alkaline Phosphatase)
Substrate: BCIP/NBT Ready-to-use solution
Blocking agent: Bovine Serum Albumin (BSA)
TNF-α is a multifunctional cytokine involved in both innate and adaptive immunity. It is produced primarily by activated macrophages, T cells, and Natural Killer cells, during immune responses, but can also be produced by other cell types, including neutrophils, mast cells, and endothelial cells. As a proinflammatory cytokine, TNF-α plays a crucial role in the inflammatory response. It activates endothelial cells, promoting the recruitment of immune cells to sites of infection or injury, and enhances the microbicidal activity of macrophages. TNF-α regulates immune responses by modulating the activity of various immune cells, including T cells and antigen-presenting cells. It is critical for both innate and adaptive immunity against a wide range of pathogens, including viruses, bacteria, and parasites, due to its ability to induce apoptosis in infected cells and enhance the production of other inflammatory cytokines. TNF-α also stimulates the production of acute-phase proteins and other mediators of inflammation, contributing to the systemic effects observed during severe infections and chronic inflammatory diseases. Aberrant TNF-α expression is associated with a variety of inflammatory and autoimmune conditions, including rheumatoid arthritis, inflammatory bowel disease, and psoriasis, highlighting its significance in immune regulation and potential as a therapeutic target.  

The Human TNF-α Enzyme-Linked ImmunoSpot (ELISpot) assay is a highly sensitive method used to detect and quantify individual cells that secrete specific cytokines or other soluble molecules. This assay is utilized in immunology to monitor cellular immune responses at the single-cell level and reliably detects and measures human TNF-α secretion by stimulated effector cells. 8-well strip (12×8) ELISPOT plates offer a flexible, cost-effective alternative to full 96-well plates by allowing smaller-scale assays, reduced reagent use, and easier handling for variable experimental setups.

Description

TNF-α is a multifunctional cytokine involved in both innate and adaptive immunity. It is produced primarily by activated macrophages, T cells, and Natural Killer cells, during immune responses, but can also be produced by other cell types, including neutrophils, mast cells, and endothelial cells. As a proinflammatory cytokine, TNF-α plays a crucial role in the inflammatory response. It activates endothelial cells, promoting the recruitment of immune cells to sites of infection or injury, and enhances the microbicidal activity of macrophages. TNF-α regulates immune responses by modulating the activity of various immune cells, including T cells and antigen-presenting cells. It is critical for both innate and adaptive immunity against a wide range of pathogens, including viruses, bacteria, and parasites, due to its ability to induce apoptosis in infected cells and enhance the production of other inflammatory cytokines. TNF-α also stimulates the production of acute-phase proteins and other mediators of inflammation, contributing to the systemic effects observed during severe infections and chronic inflammatory diseases. Aberrant TNF-α expression is associated with a variety of inflammatory and autoimmune conditions, including rheumatoid arthritis, inflammatory bowel disease, and psoriasis, highlighting its significance in immune regulation and potential as a therapeutic target.  

Application

The Human TNF-α Enzyme-Linked ImmunoSpot (ELISpot) assay is a highly sensitive method used to detect and quantify individual cells that secrete specific cytokines or other soluble molecules. This assay is utilized in immunology to monitor cellular immune responses at the single-cell level and reliably detects and measures human TNF-α secretion by stimulated effector cells. 8-well strip (12×8) ELISPOT plates offer a flexible, cost-effective alternative to full 96-well plates by allowing smaller-scale assays, reduced reagent use, and easier handling for variable experimental setups.

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Frequently Asked Questions

Virax Biolabs develops and provides immunology-profiling solutions, with a focus on T cell diagnostics and immune monitoring. Their offerings include research-use products (e.g., peptide pools, ELISpot/FluoroSpot kits, high-affinity antibodies) as well as custom immunology services (such as recombinant antibody engineering, peptide synthesis and protein sequencing).

Virax Biolabs offers a comprehensive range of products to support immunology research and diagnostic development. Their primary product categories include pathogen-specific peptide pools that enable T cell stimulation and immune-response analysis, ELISpot and FluoroSpot kits designed for measuring cytokine secretion and other immune markers, and high-affinity antibodies suitable for use in assays such as ELISA, flow cytometry, and ELISpot. Together, these products provide researchers with precise tools for profiling immune responses in various disease and vaccine studies.

Virax Biolabs provides a range of custom immunology services to meet the diverse needs of academic and industry clients. These services include recombinant antibody engineering, which involves antigen discovery, sequencing, formatting, and bio-conjugation. They also offer custom peptide synthesis and protein sequencing using advanced mass spectrometry, as well as laboratory services that support ELISA, ELISpot, FluoroSpot, and flow cytometry assays. These custom services are designed to help researchers accelerate discovery and optimize immune-response studies.

Virax Biolabs primarily serves researchers and organizations engaged in immunology, infectious disease, and vaccine development. Their customer base includes academic laboratories, biotechnology companies, and pharmaceutical firms that require high-quality immune-profiling tools and diagnostic support. By providing both off-the-shelf research products and tailored immunology services, Virax Biolabs enables scientists to investigate T cell activity, evaluate vaccine efficacy, and develop new diagnostic platforms.

Virax Biolabs’ headquarters is located at BioCity Glasgow, Bo’Ness Road, Newhouse, Lanarkshire, ML1 5UH, United Kingdom. Inquiries can be made through the “Contact” section of their website at viraxbiolabs.com.

Many of Virax Biolabs’ products, including peptide pools and ELISpot kits, are intended for research use in immune-profiling and in-vitro studies. However, the company is also developing in-vitro diagnostic (IVD) platforms and T cell diagnostics for applications such as chronic inflammation and immune exhaustion. The intended use and regulatory status vary by product, so customers should refer to the specific product details or contact the company directly for clarification.

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